Abstract
This chapter presents the application of Mössbauer spectroscopy to hemoglobin studies. The Mössbauer effect (ME) can be used to study specific regions or sites on the molecule that are of primary importance to its biological function. It allows the study of changes in this environment as the molecule goes through the various steps of fulfilling its biological function. All Mössbauer experiments are based on the utilization of this extremely well-defined energy to measure small energy differences. There are about 100 isotopes of 43 elements that have shown the effect, of which by far the most widely used is 57 Fe. It is a coincidence that iron is also important biologically. This coincidence allows the study of heme proteins, iron–sulfur proteins, iron-transport and storage proteins, and other iron-containing biological systems.
Published Version
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