Abstract

Di(2-ethylhexyl)phthalate (DEHP) have been used to increase the flexibility of the poly vinyl chloride (PVC) plastic products, including food packaging, children’s toys and medical devices. It can be absorbed into human body via air and water through burning and burying the plastic products. In this study, we investigated the up-regulated effect of DEHP on type I hypersensitivity and the inhibitory effect of grape seed extract (GSE) on DEHP-induced mast cell degranulation and related signaling cascade. Our experiment evaluated the up-regulation of degranulation, intracellular Ca2+ level, reactive oxygen species (ROS), mitogen-activated protein kinase (MAPK) and TH2-associated cytokine expression in DEHP-treated RBL-2H3 cells. DEHP also induced interleukin (IL)-4, IL-5 and transcription factor c-Maf on EL-4 T lymphoma cells. Our result revealed that grape seed extracts (75~100 μg/ml) decreased the DEHP-mediated release of histamine and β-hexosaminidase in RBL-2H3. We also found GSE inhibited the intracellular Ca2+ level, MAPK phosphorylation on DEHP-treated RBL-2H3, which result in the down-regulation of IL-4 and IL-13 mRNA expression. In conclusion, DEHP can promote type I hypersensitivity, and we speculated that GSEs might inhibit allergic responses caused by DEHP.

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