Abstract
Parainfiuenza virus type 3 (PIV-3) and bovine rhinovirus type 1 (PRV-1) were isolated simultaneously from nasal discharge samples from five of 28 cows involved in 3 outbreaks of bovine respiratory disease over a period from 1974 to 1980. They were separated from each other by a method of simultaneous application of critical dilution and immune serum, inoculation into embryonated hen's eggs, and ether treatment of samples presumed to be of mixed infection.An antibody survey revealed a significant increase in titer of antibodies against both viruses incows from which these viruses had been isolated simultaneously.
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