Abstract
Publisher Summary This chapter discusses the principles and procedures for serotyping of Escherichia coli (E. coli) and discusses the morphology and immunochemistry of the surface structures that are important in serotyping. The genus Escherichia is one of the genera of tribus Escherichieae as defined in Bergey's Manual of Determinative Bacteriology. It contains one species E. coli, which consists of Gram-negative, peritrichously flagellated rods that conform to the family Enterobacteriaceae. E. coli can be differentiated from other genera in the tribus Escherichieae by indole production, the fermentation of lactose, negative reactions in KCN, gelatin, and malonate tests. E. coli is methyl red positive, Voges–Proskauer negative, and urease negative. E. coli group is heterogeneous and contains a very high number of stable subtypes. Therefore, it will be a difficult task to command all available methods in a single laboratory, if a complete typing of strains is needed.
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