Abstract
CRES is an inhibitor of a specific member of an enzyme family called serine proteases. It is thus thought to play a role in enzymatic regulation. It has been shown to be expressed only in the testes, epididymis, sperm, and gonadotropic cells of the anterior pituitary gland. Localization of CRES in sperm is an important step in determining its role within sperm. Previous studies have shown CRES to be localized in the mouse acrosomal cap: a vesicle on the tip of the sperm head which releases its contents upon initial sperm‐egg contact. The present study shows that developmental analysis of spermatogenesis via immunohistochemistry is incompatible with the conclusion that CRES is localized in the acrosomal cap. CRES expression begins in the elongating spermatid stage of sperm development; by this point, the acrosomal cap has been completed and it has never been shown that further additions to it can occur. Western blot and immunogold electron microscopy shows that CRES is localized in the sperm tail. It is also possible that further CRES is placed on the outer sperm membranes on the head as it travels through the epididymis, which is a part of the male reproductive tract.
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