Abstract
The technique of two-dimensional (2D-) fluorescence spectroscopy covers a wide range of excitation and emission wavelengths. Several biogenic fluorophors (proteins, vitamins and coenzymes) can be detected simultaneously. The development of an optical sensor for on-line measurement of 2D-fluorescence spectra at biotechnological processes is described. This sensor is very well suited for in vivo measurements, because it is non-invasive and the on-line data can be performed in-situ using an optical light guide system. This instrumentation was used at cultivations of Saccharomyces cerevisiae to show the potential and also the reliability of 2D-fluorescence spectroscopy for bioprocess monitoring. Changes in cell metabolism (aerobicanaerobic transitions), cell growth, medium composition and also turbidity could be detected.
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