2-Deoxyglucose uptake patterns in response to pure tone stimuli in the aged rat inferior colliculus

Abstract

The tonotopic map of the inferior colliculus (IC) of aged rats (25 months old) was examined to determine whether age-related changes known to occur in the cochlea are reflected in the 2-deoxyglucose (2-DG) uptake pattern of the IC. Because aged animals have hearing losses, auditory brainstem response thresholds were measured. Animals with threshold shifts of no greater than 30 dB relative to young animals were used. Animals were injected with radiolabeled 2-DG and stimulated with continuous pure tones presented at 70 dB above the behavioral thresholds for young animals at either 1, 4 or 32 kHz for one hour in a sound attenuated booth. The stimulus sound pressure levels were chosen to achieve comparable sensation levels between the young and aged animals. The tonotopic map of the IC in aged rats was different from that reported previously for young animals (Ryan et al., 1988), in that, the regions stimulated by 1 and 4 kHz were shifted towards the higher frequencies and the uptake areas were twicc as broad for the aged animals as for the young animals. The observed 2-DG uptake patterns are consistent with an activation pattern of a high intensity stimulus and a loss of responsive elements in the cochlear apex. Similar broad and shifted bands of activated tissue may contribute to difficulties in auditory perception in aged humans with increased thresholds and sound amplification.