2-deoxy-D-glucose stimulates acid secretion from chicken proventriculus.

Abstract

Effects of 2-deoxy-D-glucose (2DG) and insulin on gastric acid secretion and blood glucose level were examined to clarify the mechanism of acid secretion in chicken. Proventricular lumen of urethane-anesthetized chicken was continuously perfused with a dilute solution of sodium hydroxide and the change in the pH of effluent was monitored as an indicator of acid secretion. 2DG (50-200mg/kg, i.v.) continuously lowered the pH level with a latent period of 30-60 min and slightly elevated the blood glucose level. By contrast, insulin (4 I.U./kg, i.v.) decreased the blood glucose to a half of the control level without changing the effluent pH. The duration of pH fall induced by 2DG was dose-dependent. The pH fall induced by 2DG was inhibited by an injection of pyruvic acid (80mg/kg, i.v.). The pH fall was abolished by bilateral vagotomy at the cervical region and abolished by the administration of atropine (0.5mg/kg, i.v.), proglumide (200mg/kg, i.v.) or metiamide (0.5mg/kg, i.v.). These results suggest that 2DG, which inhibits glucose methabolism, activates the cephalic phase through vagus nerve and thus increase cholinergic, gastrinergic and histaminergic acid secretions.