Abstract
Cold-adapted strain of Geomyces pannorum P11 was found to mineralize of phosphorus–carbon bond-containing compound—2-aminoethylphosphonic acid (2-AEP, ciliatine). The biodegradation process proceeded in the phosphate-independent manner. Ciliatine-metabolizing enzymes' activity was detectable in cell-free extracts prepared from psychrophilic G. pannorum pregrown on 4 mM 2-AEP. Phosphonoacetaldehyde hydrolase (phosphonatase) activity in a partially purified extract was demonstrated at 10 °C.
Highlights
Phosphorus is an essential element for many biomolecules and plays an important role in various biological processes
Cold-adapted strain of Geomyces pannorum P11 was found to mineralize of phosphorus–carbon bond-containing compound—2-aminoethylphosphonic acid (2-AEP, ciliatine)
For the first time, we report bioconversion of 2-AEP, which is driven at 10 °C, by a psychrophilic fungal strain of genus Geomyces
Summary
Phosphorus is an essential element for many biomolecules and plays an important role in various biological processes. The first reaction reported as transamination is carried out by 2-AEP transaminase and leads to the formation of phosphonoacetaldehyde and corresponding amino acid. This enzyme [EC 2.6.1.37] has been isolated from Pseudomonas aeruginosa [9] and Salmonella typhimurium [6, 16]. The step includes hydrolytic cleavage of the C–P bond within the phosphonoacetaldehyde molecule and results in formation of inorganic phosphate and acetate. This reaction is carried out by an enzyme phosphonoacetaldehyde hydrolase (phosphonatase) [EC 3.11.1.1] that has been purified from Bacillus cereus [24, 30]. The literature data concerning the phosphate starvation-independent ciliatine biodegradation are scarce [18, 28]
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