Abstract
The specific binding of [ 3H]rauwolscine to rat nasal mucosa membranes was saturable, stereoselective and of high affinity. The Scatchard plot pointed to a homogeneous population of binding sites (K d = 3.6 ± 0.6 nM; B max = 5.1 ± 0.7 pmol/g). The non-specific binding appeared to be non-linear, probably due to filter binding. Inhibition of [ 3H]rauwolscine binding with the subtype-selective antagonist, prazosin, suggested the presence of α 2-adrenoceptor subclasses in rat nasal mucosa. The (−)-epinephrine inhibition curves demonstrated high- and low-affinity agonist binding sites. A monophasic (−)-epinephrine inhibition curve was obtained in the presence of guanine nucleotides.
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