Abstract
A rapid method for quantitation of lytic polysaccharide monooxygenase (LPMO) in multienzyme cocktails produced by mutant strains of filamentous fungi is developed. In this method, 2,6-dimethoxyphenol and hydrogen peroxide are used as cosubstrates in a nonspecific LPMO-catalyzed reaction leading to the formation of a colored product. With this method, we determine the LPMO content in the multienzyme cocktails produced by Penicillium verruculosum recombinant strains that homologously express LPMO. The results agree closely with the findings obtained using a method based on the fine chromatographic fractionation of the cocktails followed by the qualitative and quantitative evaluation of their content by electrophoresis and mass-spectrometry and protein quantitation assays in fractions collected from chromatographic separation.
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