Abstract
2,4-dinitrotoluene (2,4-DNT) is a common environmental pollutant, and was classified as a group 2B human carcinogenic compound by the International Agency for Research on Cancer. This study determined the toxic effects of 2,4-DNT exposure on zebrafish at the embryo-larvae stage, in terms of organ morphogenesis and the expression pattern of selected target genes related to lipid metabolism and oxygen transportation. The results showed that the 120-h post-fertilization LC50 of 2,4-DNT was 9.59 mg/L with a 95% confidence interval of 8.89–10.44 mg/L. The larvae treated with 2,4-DNT showed toxic symptoms including smaller body, less skin pigment production, yolk malabsorption, and disordered liver development. Further studies on the expression of genes related to lipid transport and metabolism, and respiration indicated that they were significantly affected by 2,4-DNT. It is concluded that 2,4-DNT exposure perturbed liver development and yolk absorption in early-life zebrafish, and disturbed the lipid metabolism /oxygen transport gene expression.
Highlights
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
As a group 2B human carcinogenic agent, 2,4-DNT was studied for its toxic effect on zebrafish larvae from 24 to 120 h postcarcinogenic agent, 2,4-DNT was studied for its toxic effect on zebrafish larvae from 24 to 120 h fertilization
The results indicate that exposure to 2,4-DNT induced disordered liver development, which was closely associated
Summary
Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. 2,4-dinitrotoluene (2,4-DNT) is a common environmental pollutant, and was classified as a group 2B human carcinogenic compound by the International Agency for Research on Cancer. This study determined the toxic effects of 2,4-DNT exposure on zebrafish at the embryo-larvae stage, in terms of organ morphogenesis and the expression pattern of selected target genes related to lipid metabolism and oxygen transportation. It is concluded that 2,4-DNT exposure perturbed liver development and yolk absorption in early-life zebrafish, and disturbed the lipid metabolism /oxygen transport gene expression. Exposure to 2,4-DNT can induce cancer in laboratory animals and may result in cancer in humans, as 2,4-DNT was classified as a group 2B human carcinogenic compound (possibly carcinogenic to humans) by the International Agency for Research on Cancer of the World
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