Abstract
2,3‐Diphosphoglyceri c Acid Increase in Carotenoid Treated Atherogenic Rabbits Jeffrey Jordan Andry Diminished oxygen diffusivity in blood may be induced by hypercholesterolemia in rabbits and is thought to play a major role in the increased arterial permeability that supports atherogenesis. Hypercholesterolemic rabbits treated with the carotenoid Crocetin (CR) (extracted from saffron) for at least 4 months, has been found to increase oxygen diffusivity, lower blood cholesterol (CHO) and reduce atherogenesis. This short term study focuses on the ability of Crocetin and B Carotene (BC) treatment of hypercholesterolemic rabbits to alter their blood levels of 2,3‐Diphosphoglyceric Acid (DPG) , a component of the glycolytic sequence which causes oxygen release from hemoglobin. Nine New Zealand Strain, male rabbits (1.4 Kg av wt ) were fed an Atherogenic Diet (AD) , ad libitum, of 92% Purina Rabbit Chow, 6% Corn Oil and 2% Cholesterol, for 5 weeks. The animals were divided into 3 equal groups and injected im. on alternate days as follows: I. Control (CO) Group ‐ 0.2 ml saline, II. CR Group ‐ 2mg. CR/ 0.2ml saline and III. BC Group‐ 2mg BC/0.2ml saline. DPG assays on whole blood samples after 3 weeks of treatment averaged an increase of 8.6% DPG in blood of CR treated rabbits vs CO values. After 5 weeks, of treatment, the average DPG level was 31.9% higher in the blood samples of CR treated rabbits, and for BC samples 2.6% higher compared to the CO DPG value. Average CHO assays of blood plasma gave substantially higher values in the carotenoid treated rabbits ( 36.1% for CR and 31.5% for BC assays) compared to the CO plasma values. The increase in 2,3 DPG in the blood of CR treated rabbits vs CO assays was significant after only 5 weeks of study despite substantial hypercholesterolemia found in that group. There was slightly lowered early atherogenesis found on examination of the aortae of the CR group vs CO aortae at autopsy.
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