2,3,7,8-Tetrachlorodibenzo-p-dioxin: Genotoxicity and Oxidative Damage Potential in Human Peripheral Blood Lymphocytes

Abstract

Dioxin-like compounds, e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzo-furans (PCDFs) and dioxin-like polychlorinated biphenyls (PCBs) are a widespread and diverse group of persistent, lipophilic and hazardous environmental pollutants. Additionally, because they are by-products of chlorine- containing manufacturing process and incineration, they represent a serious environmental problem. In this research study; we investigated the genotoxic and oxidative effects of TCDD using single cell gel electrophoresis/COMET assay and measuring levels of catalase, superoxide dismutase enzymes, and malondialdehyde values for lipid peroxidation in peripheral blood lymphocyte cultures at three different doses. Blood samples were taken from healthy non-smoking male subjects by venipuncture. In this study, the three doses of 2,3,7,8-tetrachlorodibenzo-p-dioxin were used as 62.5 ng/ml, 31.25 ng/ml, 15.625 ng/ml. In comet assay, two different parameters were evaluated. Damaged cell percent (DCP). Genetic damage index (GDI). Both GDI and DCP significantly increased in a dose-dependent manner at statistical level. There is no statistically significant difference the levels of catalase, superoxide dismutase enzymes, malondialdehyde values compared with negative control.