Abstract
Selection of specific cells based on multiple factors is an essential process for clinical diagnosis/treatment and biological research. In this study, we propose a simple microfluidic cell separator based on two factors (size and surface marker), by combining HDF and magnetophoresis. Cells conjugated with immunomagnetic particles are sorted according to size due to the principle of HDF. Continuously, cells are subjected to the magnetic field, and cells conjugated with a larger number of immunomagnetic particles move downward greater than those labeled with less particles. Consequently, cells are continuously sorted based on the two factors. In the experiment, a mixture of JM cells having CD4, and HeLa cells without CD4 were prepared, and anti-CD4 magnetic immunoparticle were added. As a result, we confirmed that the enrichment/depletion of specific cells was possible, and relatively rapid cell sorting was achieved.
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