1P1-L05 オープンチップの気液界面制御による希少細胞の自動分離・回収

Abstract

For molecular analysis of the rare cells, such as Circulating Tumor Cells (CTCs), the single rare cell isolation from the suspension which contains large population of other cells is required. Here, we propose a platform which utilizes surface tension and suction force to array single cells on the microfluidic chip with open channels. Cells flowed to the meniscus of air/liquid interface due to the suction force caused by a suction pump. Then, through surface tension which was generated by the air-liquid interface, the cells were deposited and trapped in the specified pockets surrounded with micropillars. Previously we already succeeded in isolating the rare cell with high speed. We developed a system for cell isolation and pick-up. We purpose to control the position of the air-liquid interface balancing supply and suction pumps. The experiment was performed with human gastric carcinoma cell line (GCIY) cells experiments. By using our isolation methods, the trap rates of cells were nearly 90 %. Also, we utilized mouse CTC model to demonstrate clinical utility of our method. Our platform will be useful for single rare cell analysis because the collected cells could be picked up by glass micro pipette rapidly and easily.