Abstract
A rapid construction process is necessary for building up numerous cell modules into three-dimensional (3D) tissues that retain the tissue geometries and initial conditions of the cells. We proposed a new 3D assembly technique using water transfer printing to fabricate a hollow tubular tissue structure. By utilizing this assembly technique, we discuss the relationship between the 3D transcriptional body of a gel matrix and the developed shape of transferred tissue, and then fabricate a hollow tubular tissue. In this work, we demonstrated that multilayered tissues could rapidly assemble into aligned tubular tissue in the appropriate geometrical conditions using engineering approaches. We succeeded in assembling 3D multilayered neonatal normal human dermal fibroblasts tissue into a tubular construct. This simple method is applicable to many different cell types and can be used to create customized, macro-scale, 3D tissue constructs with micro-scale control of cellular alignment. These results show that this method could be potentially useful for creating in vitro simulators for drug efficiency evaluation and operative training.
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