奈米矽線場效電晶體在生化研究的應用:1.極高靈敏度且具多次使用性之H5N2禽流感病毒偵測器2.神經/電晶體介面整合

Abstract

The bulk of this master thesis is dedicated in studying various biological issues by silicon nanowire field-effect transistor. This study can be divided into two major part: In the first part, we aim to establish a reusable and highly sensitive H5N2 avian influenza virus sensor by disulfide bond based surface modification method. Currently, we are able to detect virus sample of concentration ranging from picomolar to attomolar. The sensitivity and reusability is proven to be genuine through many control experiments, including fluorescence imaging, atomic force microscopy and electrical measurement from silicon nanowire field-effect transistor device. From experimental observation we found that device can detect virus sample even under relatively short debye length condition. We attribute the reason for such sensitivity to the random orientation of surface modified antibody. We also find that signal collection time of measured data is apparently much faster than predicted by convection diffusion mass transfer model. Electrophretic migration is proposed here to account for the enhanced mass transfer effect. As for the second part of the thesis, we aim to develop a technique that can precisely interface the neuron cells with silicon nanowire field-effect transistor active sensor area. Physical and chemical surface modification is applied to increase the hydrophilicity of poly(dimethyl)siloxane (PDMS). PDMS is first treated with 10W, 42 seconds of oxygen plasma, and then react with 3-amino(propyl)trimethoxysilane. The increase in PDMS hydrophilicity is measured by contact angle measurement and X-ray photoelectron microscopy. According to the results, surface modified PDMS possess moderate hydrophilicity which is suitable for subsequent cell culturing.