1H NMR study on the interaction between trans-Cl 2Pd(dipeptide) 2 (dipeptide is glyglyOH and gly-L-nvalOH) and the dodecanucleotide [d(CGCGAATTCGCG)] 2

Abstract

The metal binding properties of trans-Cl 2Pd(glyglyOH) 2 and trans-Cl 2Pd(glynvalOH) 2 towards the oligonucleotide dodecamer [d(CGCGAATTCGCG)] 2 duplex, have been studied by 1D and 2D 1H NMR spectroscopy. A selective binding of the metal with G 4, followed by G 2 was observed with both complexes. This indicates that an intrastrand GNG(G 2C 3G 4)(N 7-N 7) chelation of Pd(II) with the dodecamer duplex may have taken place. The Pd(II)-duplex interaction is less aggressive with trans-Cl 2Pd(glynvalOH) 2, than with trans-Cl 2Pd(glyglyOH) 2. NOE connectivities in the first case show a through space hydrophobic ligand–ligand interaction of the dipeptide and oligonucleotide protons.