Abstract
A study was performed on human urinary glycosaminoglycans (GAGs) by one- and two-dimensional 500MHz proton nuclear magnetic resonance (1H-NMR) spectroscopy. Urinary GAGs were separated by the precipitation methods with hexadecyl pyridinium and with 85% ethanol, and purified by the β-elimination reaction in alkaline medium containing sodium borohydride to remove proteins and/or peptides. The spectrum of each purified GAG sample from healthy human urine which included chondroitin sulfates (ChS), dermatan sulfate (DS) and heparan sulfate (HS) showed the characteristic signal(s) corresponding to each GAG chain. The assignments and integrations of the structure specific signals in the spectrum made it possible to perform qualitative and quantitative analyses on human urinary GAG mixtures. Furthermore, analytical results of urinary GAGs in children, adults and the aged indicate that the present method is applicable to investigate the effect of aging on excretion of urinary GAGs.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
