Abstract
From field harvest to cold room storage, rockmelon accumulates heat from the high ambient temperature which accelerates the metabolic changes and shortens the market life of the fruit. Hydro-cooling treatment has been used to reduce the temperature of fresh produce after harvest in order to maintain quality during postharvest storage. The purpose of this study was to elucidate the influence of hydro-cooling on phytochemical content and metabolite changes in the rockmelon. In this study, hydro-cooling application has effectively preserved the beta-carotene of rockmelon pulp. The results also suggested that high phenolic content in rind and pulp of rockmelon was highly maintained by using 1/2 cooling time (CT). A discriminatory analysis on the metabolites responsible for the variation between weeks of storage (WS) at each CT of rockmelon was successfully achieved by using 1H NMR-based metabolomics. The principal component analysis scores showed a noticeable and clear separation into four clusters representing the WS 0, 1, 2 and 3 for 0 and 3/4 CT rind extracts. Various assigned 1H NMR chemical shifts referring to the metabolites responsible for the variation were ascribed. Among the identified metabolites, the aromatic amino acids such as phenylalanine, tryptophan and tyrosine were not changed significantly during WS 0, 1 and 2 as they were grouped into the same cluster in 1/2 CT hydro-cooled rind extracts.
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