1H, 7Li and 133Cs multicomponent self-diffusion NMR study on ion binding of Li + and Cs + to nucleotides and aggregation of nucleotides in aqueous solution

Abstract

The Fourier transform NMR pulsed-gradient spin-echo self-diffusion technique was used for studies of nucleotides (AMP, CMP, GMP and UMP) with Li + or Cs + added, in 2H 2O. 1H-, 7Li- and 133Cs-NMR-based self-diffusion data on the constituents provide a picture of both the degree of ion binding to nucleotides and the self-association of nucleotides in aqueous solution. Self-diffusion coefficients were investigated in a concentration range up to 0.3 molal nucleotide in 2H 2O, while keeping the metal ion concentration of Li + or Cs + at twice the nucleotide concentration throughout the investigations. The self-association studies reveal that the aggregation constants of the Li salts differ only slightly from the corresponding constants for the disodium salts of the mononucleotides. Within a two-site bound-free model for the counterions and a cooperative indefinite aggregation model for the nucleotides one finds that the degree of ion binding for all these nucleotide systems remains approximately constant, in spite of increasing aggregate concentration. This corresponds to the well-known polyelectrolyte ion condensation behaviour, indicating that large aggregates are formed, supporting previous findings by the present authors on the aggregation behaviour of nucleotides. An observed large effect on the 17O relaxation of water in nucleotide systems can only be reconciled with the presence of relatively large aggregates in solution.