1H, 15N chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNALeu (CAG)

Abstract

tRNA molecules are the adaptors in ribosome-based protein biosynthesis and are stabilized by Mg(2+). However, the detailed mechanism for the Mg(2+) mediated stability is not fully understood. To study the effects of Mg(2+) on conformational flexibility of Escherichia coli tRNA(Leu) (CAG) at millisecond timescale, we applied NMR spectroscopic approach to measure proton exchange rates of imino groups in the presence of different concentration of Mg(2+) and correlated them with the corresponding aminoacylation activity of tRNA(Leu). Here, we report the first part of the above mentioned study, the (1)H, (15)N chemical shift assignments of the imino groups in all base pairs of Escherichia coli tRNA(Leu) (CAG) based on 2D (1)H-(15)N TROSY, 2D NOESY and 3D NOESY-HMQC experiments. This work laid the foundation for the NMR study of tRNA(Leu) (BMRB deposits with accession number 17078).