Abstract
SMARCAD1 is a non-canonical chromatin remodelling ATPase, unique in its domain organization in that is encodes tandem ubiquitin binding CUE domains along with a classical SNF2 helicase ATP-dependent motor. SMARCAD1 is conserved from yeast to humans and has reported roles in the maintenance of heterochromatin following replication and in double-strand break repair. Here we present the 1H, 13C and 15N assignments for the tandem CUE domains and for the disordered regions that flank them. These assignments provide the starting point for detailed investigations of the structure and interactions of this region of SMARCAD1.
Highlights
SMARCAD1 is a non-canonical member of the SNF2 family of chromatin remodelling ATPases, unique in its domain organization encoding two CUE domains along with a classical SNF2 helicase ATP-dependent motor (Okazaki et al 2008; Schoor et al 1999; Soininen et al 1992)
SMARCAD1 belongs to the Swr1-like subfamily, which is evolutionarily the most conserved class of chromatin remodelling ATPases, from yeast to humans
SMARCAD1, in association with PCNA and transcriptional repressors KAP1, histone deacetylases HDAC1/2 and histone methyltransferase G9a/GLP, is a key factor required for the re-establishment of repressive chromatin structures following replication (Mermoud et al 2011; Rowbotham et al 2011)
Summary
SMARCAD1 ( known as Etl-1, HEL-1 or KIAA1122) is a non-canonical member of the SNF2 family of chromatin remodelling ATPases, unique in its domain organization encoding two CUE domains (previously characterized as mono-ubiquitin binding domains) along with a classical SNF2 helicase ATP-dependent motor (Okazaki et al 2008; Schoor et al 1999; Soininen et al 1992). The members of the CUE domain family have been characterized as mono-ubiquitin binding domains; sequence conservation is low both between CUE1. Sequence conservation is low amongst key residues involved in ubiquitin recognition suggesting a reduced affinity for the canonical substrate. To gain an understanding of the molecular mechanism by which SMARCAD1 employs its CUE domains to establish protein–protein interactions, we have embarked on an NMR spectroscopy study to characterise their structure and dynamics. The second, eCUE, corresponds to only the first CUE domain and the disordered region preceding it (residues 109–206)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
