Abstract
KKT4 is a kinetoplastid-specific microtubule-binding kinetochore protein that lacks significant similarity to any known kinetochore or microtubule-binding proteins. Here we present the 1H, 13C and 15N resonance assignments for several fragments from the microtubule-binding domain of KKT4 (KKT4115–343) from Trypanosoma brucei. These assignments provide the starting point for detailed investigations of the structure, dynamics and interactions of the microtubule-binding region of KKT4.
Highlights
A key structure involved in this process is the kinetochore, a dynamic protein complex that assembles onto centromeric DNA of each chromosome and captures spindle microtubules (Brinkley and Stubblefield 1966; Cheeseman 2014)
None of the canonical kinetochore proteins has been found in kinetoplastids, an evolutionarily divergent group of unicellular flagellated eukaryotes including parasitic trypanosomatids (e.g. Trypanosoma brucei, Trypanosoma cruzi, and Leishmania species)
No significant similarity to conventional kinetochore proteins is found in these proteins and very little is known about their structure, function and interactions
Summary
Cells must accurately transmit their genetic material into two daughter cells in an organised manner. A key structure involved in this process is the kinetochore, a dynamic protein complex that assembles onto centromeric DNA of each chromosome and captures spindle microtubules (Brinkley and Stubblefield 1966; Cheeseman 2014). These NMR assignments provide the starting point for detailed investigations of the structure and dynamics of KKT4
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