Abstract
A local pH sensing method in a microchip using a functional gel-microtool was newly developed. We used a salting-out gel-microtool impregnated with BromoThymol Blue (BTB), which is used as a pH indicator. The salting-out gel-microtool is made of ENT-3400, which is hydrophilic photo-crosslinkable resin. The primary constituent of this resin is polyethyleneglycol. A solution mixed with ENT-3400, BTB, and high concentrations of electrolytic solution are stirred, and a lot of microspheres impregnated with BTB are obtained. These microspheres are cured by UV illumination and adhere to the glass substrate, however, we can manipulate them one by one using the optical tweezers. Therefore, we can move and position it in the closed environment. Here we propose to use the salting-out gel-microtool with BTB as a carrier of pH indicator. We can measure pH value locally from the color of microtool using calibrated color information in YCrCb color space. We succeeded in measuring local pH value with the pH sensing gel-microtool by locating it at the desired point in the microchip.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
