Abstract
Summary 19F nmr relaxation proved to be a proper method to evaluate the Cu2+/Cu+ ratio at the active site of Cu, Zn superoxide dismutase in either equilibrium or turnover conditions. In the steady-state under fluxes of O2−, the enzyme was found to contain 50% Cu2+, in accord with the equal rates of copper catalytic reduction and oxidation. Previous results giving 75% Cu at the steady-state without change of the overall catalytic constant were confirmed for samples subjected to freeze-drying or freezing-thawing.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
