1967 THE INFLUENCE OF ERYTHROCYTE BAND III ANION TRANSPORTER ON THE OXALATE TRANSPORT OF CALCIUM OXALATE MONOHYDRATE STONE FORMERS VS. NORMAL CONTROLS

Abstract

You have accessJournal of UrologyStone Disease: Basic Research1 Apr 20101967 THE INFLUENCE OF ERYTHROCYTE BAND III ANION TRANSPORTER ON THE OXALATE TRANSPORT OF CALCIUM OXALATE MONOHYDRATE STONE FORMERS VS. NORMAL CONTROLS Sven Oehlschläger, Susanne Fuessel, Axel Meye, Jana Herrmann, Ulrike Lotzkat, Steffen Albrecht, and Manfred Wirth Sven OehlschlägerSven Oehlschläger More articles by this author , Susanne FuesselSusanne Fuessel More articles by this author , Axel MeyeAxel Meye More articles by this author , Jana HerrmannJana Herrmann More articles by this author , Ulrike LotzkatUlrike Lotzkat More articles by this author , Steffen AlbrechtSteffen Albrecht More articles by this author , and Manfred WirthManfred Wirth More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.1977AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Anion exchangers regulate the oxalate transport in many tissues. We examined the transmembrane erythrocyte oxalate transport, the band III transport protein levels and the concentrations of plasma, cellular and urinary oxalate to distinguish normal controls (NC) vs. calcium oxalate stone formers (COM). METHODS Blood was drawn into a 10 ml ammoniac heparinat monovette from 51 NC and 25 COM. The red cells were isolated and divided for cellular oxalate measurement and for resuspention in pH-value 8.02 buffered solution. 0.1 mmol/l oxalate was added. The supernatant immediately and one hour after incubation was mixed with 15% HCl solution for oxalate measurement to calculate transmembrane erythrocyte oxalate flux. The erythrocyte band III transport protein (SLC26a4) was determined by Western Blot analyses (Band III antibody, GAPDH as reference). Additionally plasma and urinary oxalate concentration from a single urine spot were analyzed. RESULTS The mean erythrocyte band III transport protein levels and the mean urinary oxalate concentration were significantly higher in COM. The mean cellular oxalate concentration was significantly lower in COM. The erythrocyte band III transport protein significantly correlated with the erythrocyte transmembrane oxalate flux (ph value 8.02) in both groups (r=0.25–0.53;p=0.01-0.04). CONCLUSIONS High values of band III transport protein with low transmembrane oxalate flux and low cellular oxalate in COM and inverse effects in normal controls implicate a structural and / or functional transport defect in the oxalate clearance of calcium oxalate monohydrate stone formers. NC (n=51) COM (n=25) p-value plasma oxalate (μmol/l) 7.42±0.30 6.29±0.47 n.s. cellular oxalate (μmol/l) 4.03±0.49 2.35±0.26 p<0.05 band III transport protein /RBC 4.17±0.61 8.76±2.12 p<0.02 transmembrane erythrocyte oxalate transport (pH 8.02) (μmol/l*h) 1.53±0.38 0.72±0.30 n.s. urinary oxalate (mmol/l) 0.25±0.01 0.31±0.02 p<0.04 Dresden, Germany© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e764 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Sven Oehlschläger More articles by this author Susanne Fuessel More articles by this author Axel Meye More articles by this author Jana Herrmann More articles by this author Ulrike Lotzkat More articles by this author Steffen Albrecht More articles by this author Manfred Wirth More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...