195 Proteomic Analyses of ILC2 and ILC3 from Human Skin and Peripheral Blood Reveal Distinctive Phenotypes and Functions

Abstract

Innate lymphoid cells (ILC) are important members of the skin-associated immune system. Despite their central role in healthy skin and inflammatory skin diseases, the constituent proteins of ILC have not been characterized in-depth. Here, we purified the two main human skin ILC subsets — CRTH2+ ILC2 and CRTH2–CD117+NCR2– ILC3 — from healthy skin (n=10) by flow cytometry and used state-of-the-art mass spectrometry-based proteomics for in-depth proteome analysis. For comparison, we included blood-derived ILC analogues from healthy donors in our study.