Abstract

193 – Figure 1. Left: IL-1shRNA plasmid map showing coding region for IL-1shRNA loop. Right: IL-1β expression in chondrocytes conditioned by LPS exposure (LPS) and transduced with pSIREN-IL-1shRNA (pIL-1), pIRES-puro3-IGF (pIGF), or pIRES-puro3-IGF-IL-1shRNA (pIL1sh+IGF). LPS induced a robust IL-1 response, which was significantly reduced by IL-1 RNA interference (pIL-1shRNA or pIL-1sh+IGF). Chondrocytes transduced with pIGF continued to express elevated IL-1 mRNA under the influence of LPS. to study chondrocyte mechanobiological response to complex loading patterns.

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