Abstract
This chapter focuses on the quantitative conversion of mucin-type sugar chains to radioactive oligosaccharides. The method described in this chapter can label oligosaccharide alcohols quantitatively with little excess radioactive materials. This chapter applied this method for the comparative study of the mucin-type sugar chains of human chorionic gonadotropins purified from the urine of pregnant women and of patients with various trophoblastic diseases. ∼9 Since then, the method has been applied for the quantitative analysis of mucin-type sugar chains of all glycoproteins. The percent molar ratio of each oligosaccharide is calculated from the radioactivity of the fraction. Each value is divided by the number of amino sugar residues included to obtain the molar ratio in radioactivity. The radioactivity of each peak is then converted to percent total radioactivity.
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