Abstract
Publisher Summary This chapter describes a few analytical methods used for the determination of the K vitamins. The Schilling-Dam procedure is the classical colorimetric method used for vitamin K1 (2-methyl-3-phytyl-1,4-naphthoquinone) assay. This method is based on the reaction between vitamin K1, 5-imino-3-thione-l,2,4-dithiazolidine, and potassium hydroxide in ethanol. The product is a violet-blue complex, the intensity of which is measured at 410 nm. In the case of vitamin K3 (menadione), a reddish complex is formed with maximum absorption at a wavelength of 440 nm. Vitamin K1 and vitamin K3 have been quantitatively determined by gas-liquid chromatography (GLC). When a hydrogen flame ionization detector is used, calibration plots cover a range of 0.5 to 2 μg. Phosphorimetry has been used to determine quantitatively vitamins K1 and K3. This method is based on the direct measurement of phosphorescence intensity of vitamin K1 (emission maximum at about 570 nm) and of vitamin K3. A photochemical-fluorometric method has been described for the quantitative determination of vitamin K1 and is described in the chapter.
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