19 Evaluation of Fixed-time Artificial Insemination and Estrus Expression in Angus-cross Heifers with Female Sex-sorted Semen Using 6-day and 7-day CIDR Protocols at Multiple Time Intervals

Abstract

Abstract The objective was to investigate the influence of progesterone exposure time and breeding time on fixed-time artificial insemination (FTAI) protocols using sex-sorted semen in Angus-cross heifers (n = 85; mean BW = 324 kg.). Heifers were assigned randomly into four treatment groups. Treatments 1 and 2 used a 6-day CO-synch + controlled internal drug release (CIDR) protocol with gonadotropin releasing hormone (GnRH) administration and CIDR insertion on day 0 and prostaglandin F2α (PGF) administration on day 6, upon simultaneous CIDR removal. Treatment 1 heifers underwent FTAI 72 h post CIDR removal, and Treatment 2 heifers underwent FTAI 80 h post CIDR removal. Treatments 3 and 4 used a 7-d CO-synch + CIDR protocol with GnRH administration and CIDR insertion on d 0 and PGF administration on d 7, upon simultaneous CIDR removal. Treatment 3 heifers underwent FTAI 54 h post CIDR removal, and Treatment 4 heifers underwent FTAI 62 h post CIDR removal. Estrus expression was determined using an estrus detection patch (Estrotect™, Rockway Inc., Spring Valley, WI). Estrus expression was classified as a patch with 50% or more of the color rubbed off and the absence of estrus expression as a patch with less than 50% of the color rubbed off. All heifers were inseminated using female sex-sorted semen from a single Angus sire (SexedULTRA 4M™, STgenetics, Navasota, TX). Pregnancy was determined by ultrasound 57 d following FTAI. Pregnancy rates and patch scores were analyzed using the GLIMMIX procedure of SAS 9.4 with treatment as a fixed effect. There was no effect of treatment on the first service AI rates (P = 0.91) or on estrus expression (P = 0.17) in heifers. In conclusion, the combinations of progesterone and breeding time in this study did not affect first service AI conception rates or estrus expression in beef cattle heifers.