Abstract
Publisher Summary Toluene treatment kills ceils of Escherichia coli and makes them permeable to molecules of low molecular weight. Despite the fact that these cells are no longer viable, they retain a number of their physiological functions and the ability to synthesize DNA, if supplied with the necessary substrates. This chapter describes the assay of DNA synthesis in toluene-treated cells based upon the incorporation of radioactively labeled deoxynucleotide residues into acid-insoluble polydeoxynucleotides. DNA synthesis in toluene-treated cells occurs in a normal manner if the reaction mixture contains Triton X-100, a nonionic detergent. One of the convincing arguments that DNA synthesis in the toluene-treated cell system duplicates active replication in vivo has been the temperature effect on temperature-sensitive replication mutants in vitro .
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
