Abstract
Publisher Summary This chapter discusses the development of immortalized cell lines for transplantation in central nervous system (CNS) injury and degeneration models. Cell lines can be generated using several strategies. One strategy is the culture and cloning of tumor cells, examples of which include the PC 12 cell line derived from a pheochromocytoma, the B16/C3 line derived from a murine melanoma, the IMR-32 line derived from a human neuroblastoma, and the C6 cell line cloned from a rat glial tumor. Each of these lines has been used for studies of brain transplantation. Another strategy is to produce hybrid cells from fusion of primary cells with tumor cells. Examples include two cell lines derived from rat and mouse embryonic mesencephalic cells and the murine neuroblastoma-glioma cell line N18TG2. This chapter focuses on the methodology for one of the techniques using immortalizing genes to generate cell lines. It also discusses the characterization and transplantation of these cell lines.
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