19. Biocompatibility of bimetallic FEGDO3 nanoparticles with human blood components for the development of MRI based functional imaging

Abstract

Background and Aims: Development of high contrast molecular imaging devices has gained tremendous potential in biomedical applications. Nanotechnology-based strategies have been evolved as one of the most promising field to design more appropriate and effective nanoparticles for such applications. However, biocompatibility of developed nanoparticles with human blood components is of pivotal importance for their clinical applications. In this study, we investigated the biological effects and stability of FeGdO3 nanoparticles during interactions with human blood and serum components. Methods: In order to this, human blood and platelet rich plasma samples were treated with 1 μg/mL, 2 μg/mL, 4 μg/mL and 8 μg/mL concentration of FeGdO3 nanopartcles for 30 min, 60 min, 120 min, 240 min, 24 h and 72 h in vitro. Moreover, peripheral blood mononuclear cells were also treated with similar concentrations of nanoparticles to investigate the effect of these nanoparticles in time dependent manner. Following to biocompatibility assessment of FeGdO3 nanoparticles, the dual-MRI contrast property was evaluated using 1.5 T Magnetome using different concentrations mixed with human blood. Results: Complete blood picture showed no adverse effect of tested concentrations of nanoparticles on human blood components such as platelet and RBC counts and levels of hemoglobin and HCT. Furthermore, serum stability of nanoparticles showed no significant effect at different concentrations of serum during interaction. Enhanced dual-MRI contrast was observed for FeGdO3 nanoparticles in human blood. Conclusions: In summary, the use of FeGdO3 showed high stability and biocompatibility with human blood components with increased T1 and T2 MRI-contrast which could be utilized for the future development of functional imaging in human applications. The authors have none to declare.