Abstract
Objective To evaluate the feasibility of 18F-FDG PET/CT in assessing the radiosensitizing effect of oleanolic acid (OA) in C6 rat glioma model, and to explore the possible mechanism of radiosensitizing effect of OA. Methods Thirty-two C6 glioma-bearing SD rats were divided into 4 groups by random number table: group A without OA and radiotherapy, group B with OA alone, group C with radiotherapy alone, group D with OA and radiotherapy. 18F-FDG PET/CT was performed before radiotherapy, at 24 h and 7 d after radiotherapy. The tumor SUVmax was measured. All rats were sacrificed and tumor tissues were excised for HE and immunohistochemistry staining. Paired t test, one-way analysis of variance, LSD-t test and Spearman correlation analysis were performed to analyze the data using SPSS 13.0. Results There was no statistically significant difference in SUVmax among the 4 groups (SUVmax of groups A, B, C, D: 5.252±0.536, 5.261±0.544, 5.273±0.520, 5.232±0.507) before treatment (F=0.008, P>0.05). At 24 h post-radiotherapy, SUVmax of groups C and D (4.766±0.511 and 4.403±0.486) decreased significantly (t=14.788, 13.366, both P 0.05). At 7 d post-radiotherapy, SUVmax of groups C and D decreased significantly (t=9.750, 10.530, both P 0.05). SUVmax of Group D decreased significantly compared with that of group C (t=5.329, P>0.05). Less tumor cells and more areas of necrosis were observed in group D compared with those in other groups by HE staining. The expression of HIF-1α was lower in group D than that in other groups by immunohistochemistry staining. HIF-1α expression was positively correlated with SUVmax (r=0.853, P<0.05). Conclusion 18F-FDG PET/CT has potential to evaluate the radiosensitizing effect of OA in vivo, and the radiosensitization of OA might be related to the down regulation of HIF-1α. Key words: Glioma; Radiotherapy; Radiation-sensitizing agents; Oleanolic acid; Tomography, emission-computed; Tomography, X-ray computed; Deoxyglucose; Rats
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