189 Integrating single-cell and spatial transcriptomics of human hair follicles to define transcriptional signature of follicular dermal papilla

Abstract

The follicular dermal papilla (DP) is a specialized hair mesenchymal cell that plays pivotal roles in hair follicle (HF) development and morphogenesis. Although transcriptome data exists for HF, characterization of human DPs at the single-cell level has been unsatisfactory due to the limited number of DP cells under single-cell dissociation condition. To generate a high proportion of mesenchymal cells, we manually isolated anagen HFs and DPs from three human scalp specimens and used these isolated populations for single-cell RNA sequencing (scRNA-seq).