1874-P: The Improvement of Type 2 Diabetes by DJB Is related to the Improvement of Intestinal Glucose Sensing and Glucose Transportation

Abstract

Duodenojejunal bypass (DJB) is an experimental procedure to study the mechanism of metabolic surgery in type 2 diabetes (T2DM),independent of weight changes. The aim of this study was to evaluate the role of Gastrointestinal (GI) glucose sensing and glucose transportation in DJB regulated glucose metabolism using T2DM rat model. T2DM was induced in Wistar rats with 2 months of high-fat diet and one low-dose streptozocin (STZ) injection. T2DM rats were randomly divided into sham operation group (T2DM-Sham, n=8) and DJB group (T2DM-DJB, n=8). Eight Wistar rats were used as wild type control group(Control, n=8). The results showed that DJB surgery significantly improved blood glucose homeostasis in T2DM rats. The hematoxylin-eosin staining results revealed that DJB operation thickened the wall of small intestine comparing to Sham operation. DJB also increased the length of villus in small intestine. Western Blot and qRT-PCR results showed that DJB significantly increased the expression of sodium-glucose co-transporter-1 (SGLT1) while reducing the expression of glucose transporter-2 (GLUT2) in the alimentary limb of DJB rats. The results also showed the expression of intestinal sweet receptors (T1R2/T1R3), Glucagon-like peptide 1/2 (GLP1/2) and its receptor (GLP1/2R) increased significantly in the alimentary limb after DJB surgery. Here we describe a new observation that DJB surgery lowers blood glucose level via intestinal glucose transport and glucose sensing regulation in the alimentary limb. Disclosure B. Jiang: None. H. Wang: None. W. Wang: None. Q. Yan: None. M. Qu: None. Z. Gao: None. Funding National Natural Science Foundation of China (81871892); Natural Science Foundation of Shandong Province (ZR2019BH036); Technology Development Plan of Weifang (2018YX027)