186 - Dental Pulp Mesenchymal Stem Cells for Treatment of Periodontal Disease and its Relationship with Oxidative Stress Markers

Abstract

Background Periodontal disease (PD) is a chronic disease of the tooth supporting tissues, characterized by gingival bleeding, periodontal pocket formation, destruction of connective tissue attachment and alveolar bone loss. In this sense, it has been pointed out that oxidative stress (OxS) is involving in the pathogenesis of PD. A therapeutic option to regenerate tissues for loss due to PD is the placement of mesenchymal stem cells derived from tooth pulp (MSC). MSCs generate a protective environment against OxS, in vitro, where respiratory burst is regulated downwards, thereby protecting the surrounding tissues from this damage. Experimental reports in animal models suggest that MSCs have antioxidant potential and, consequently, the ability to reduce OxS. In light of these findings, a decrease in oxidative damage in periodontal tissues can be expected after application of dental pulp MSC. Objective To determine the effect of Dental Pulp MSC on OxS and PD. Methods It was carried out a cases series of 10 patients with PD. Clinical measurements and saliva samples were collected before and after 3 and 6-months of Dental Pulp MSC application. Thiobarbituric acid reactive substances (TBARS), superoxide dismutase (SOD) and total antioxidant capacity (TAC) were spectrophotometrically assayed in saliva. Results Application of Dental Pulp MSC showed a decreased in mean of clinical parameters at 6 months (4.6±0.9 to 3.8±0.8 mm). It was also found a statistically significant decrease in TBARS at 6 months (0.0826±0.018 to 0.0381±0.042 μmol/L). Salivary SOD levels were significantly higher at 3 months (1.500±0.777 to 2.754±1.32 U/l) (p>0.05). Conclusions Our findings suggest that the treatment with DPMSC for periodontal disease has effect antioxidant. Trial registry: ISRCTN 12831118