Abstract
Objective: To construct Pik3r3 gene pancreatic beta cell knockout mice by the Cre recombinase system, and provide a more specific animal model for the study of the role and mechanism of Pik3r3 in insulin secretion. Methods: Pik3r3 flox/flox transgenic mice were constructed by CRISPR/Cas9 technology and hybridized with pancreatic beta cell specific Cre recombinase (Ins2-cre) tool mice. We identified their progeny genotype by PCR and sequencing technology. The knockout (KO) and their wild type (WT)mice were used to measure body weight, glucose tolerance, and insulin secretion level. Results: pancreatic beta cell specific Pik3r3 gene conditional knockout mice, with the genotype of Pik3r3 flox/flox/CreT, were successfully constructed. Further studies showed that Pik3r3 KO mice had impaired glucose tolerance but no significant change in body weight compared with WT mice. Conclusion: The Pik3r3 gene pancreatic beta cell knockout mouse model was successfully constructed, which provides a research platform for exploring the role of Pik3r3 gene in the development of diabetes. Disclosure X.Zhu: None. Funding National Natural Science Foundation of China (82000733)
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