18 - The Effectiveness of Different Types of Varnishes in Preventing Erosion

Abstract

The aim of this in vitro study is to evaluate the effect of fluoride varnish, bioactive glass varnish, eggshell powder and sodium tri-metaphosphate treated eggshell membrane powder containing varnish against erosion. 27 teeth were cut with a water-cooled low-speed diamond separator. Two 2×3 mm windows were created in the middle three of the buccal surfaces of the specimens and the remaining parts were covered with acid-resistant nail polish. Fluoride varnish (FV), Bioactive glass varnish (BV), STMP treated eggshell and eggshell membrane containing varnish (EV) were applied to one of the two windows in each tooth sample, the other window was used as a control. Using separate containers, samples were immersed in cola drink (6 ml/sample, p H 2.6, Coca-Cola, Ankara, Turkey) gentle shaking (Duomax 1030, Heidolph) at room temperature for 2 minutes. Then the samples were kept in 0.4 ml artificial saliva for 2 minutes. This pH cycle was repeated 4 times. The surface properties of the samples were examined in a scanning electron microscope, the Ca, P, F, Mg, K and Na ratios were determined, and the Ca/P ratio was calculated. The Ca/P ratio in the area where FV is applied is 1.56, which is lower than 1.61, the stochiometric ratio in human enamel. This ratio is 1.50 in the FV control group. The Ca/P ratio of the BV group was 1.63 and 1.51 in the BV control group, 1.63 in the EV group and 1.52 in the EV control group. No statistical difference found among groups. Also, on the enamel surface treated with BV and EV, a protective layer observed. Against dental erosion, BV and EV can be used as an alternative to fluoride varnishes.