18] Peptide synthesis using immobilized proteases

Abstract

This chapter discusses peptide coupling reactions on a preparative scale in which immobilized proteases can be used with the advantage of avoiding reaction conditions, which are normally required for chemical condensations. The simplified work-up procedure that becomes possible when immobilized proteases are used, the long-term stability of the immobilized enzyme preparations, and the successful reutilization experiments are among the advantages to such an approach. The use of enzymes should provide a biological alternative to chemical peptide synthesis. There has been a revival of interest in the ability of proteolytic enzymes to catalyze the formation of peptide bonds for the synthesis of biologically active peptides and the semisynthesis of proteins. The application of enzymes for organic synthesis is increasing rapidly in importance and has been stimulated to a considerable degree by the availability of immobilized enzymes. Proteases can be immobilized without the loss of function, and the potential of immobilized proteolytic enzymes for peptide synthesis is demonstrated in the chapter.