Abstract
The technique of E.M. Southern for transferring electrophoretically separated DNA fragments to nitrocellulose paper for subsequent hybridization with radioactive RNA or DNA probes has proved to be a powerful tool for the analysis of DNA. Until recently, the only comparable procedure for RNA has been to transfer and covalently couple it to activated cellulose paper (diazobenzyloxymethyl paper, DBM paper) according to the method of Alwine et al . Although DBM paper does offer the advantage of covalent attachment, it has several drawbacks as discussed in this chapter. The chapter also describes a related method for dotting RNA onto nitrocellulose pretreated with high salt. Thus, all the advantages that nitrocellulose blotting previously afforded for DNA analysis are now available for RNA analysis as well.
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