Abstract
<TEX>$^{18}F$</TEX> 방사성동위원소 피폭으로 금붕어 뇌에서 유도된 생리활성물질을 조사하기 위해 금붕어 뇌에 존재하는 신경전달물질의 생성 변화에 관한 연구를 수행하였다. <TEX>$^{18}F$</TEX> 580 mCi를 납으로 밀폐된 공간에 두고 선원으로부터 4 cm 떨어진 위치에 금붕어들을 수조에 넣어 4시간 동안 노출시켰다. 거리, 노출시간 및 <TEX>$^{18}F$</TEX>의 반감기로부터 계산된 흡수선량은 약 2 Gy이었다. <TEX>$^{18}F$</TEX>에 의해 피폭된 금붕어 뇌와 피폭되지 않은 정상 금붕어의 뇌를 각각 10마리씩 절개하여 즉시 냉동 보관 하였다. 각각의 조직들은 초산으로 추출하였으며, 동결건조 후 샘플들은 증류수로 녹여 HPLC를 사용하여 물질을 정제하였다. <TEX>$^{18}F$</TEX>로 피폭된 금붕어와 피폭되지 않은 금붕어의 뇌에 존재하는 생리활성물질의 흡광도의 세기는 전체적으로 커다란 차이점은 없었지만, 방사선에 노출된 금붕어 뇌 추출물의 경우 13분대에 해당하는 물질 피크만이 정상 금붕어 뇌 추출물에 비해 매우 크게 증가하였다. 분석 결과, 이 물질은 트립토판(tryptophan, Trp)으로 밝혀졌다. 따라서 금붕어의 뇌에 존재하는 신경전달물질인 트립토판은 <TEX>$^{18}F$</TEX> 방사성동위원소 피폭으로 금붕어 뇌에서 농도 변화가 유도된다는 것을 이 결과는 나타내고 있다. In order to investigate the changes in bioactive materials induced in goldfish brains by <TEX>$^{18}F$</TEX> irradiation, the variations in the neurotransmitter levels in the whole brain were studied. The distance between the goldfish and 580 mCi of <TEX>$^{18}F$</TEX> was about 4 cm, and the exposure lasted for 4 hrs. The absorption level calculated based on the distance, exposure time, and half-life of <TEX>$^{18}F$</TEX> was approximately 2 Gy. After sacrifice by <TEX>$^{18}F$</TEX> irradiation or untreated conditions, ten brains were dissected or immediately frozen, respectively. The tissues were extracted in acetic acid. After lyophilization, the samples were dissolved in distilled water and were further purified on a reverse-phase HPLC column. There were no differences in the intensities of the bioactive materials between <TEX>$^{18}F$</TEX>-exposed goldfish and control goldfish, while the only peak corresponded to 13 min, which indicated a significant increase in the irradiated brains. Our analysis has found that this compound is tryptophan. This result suggests that <TEX>$^{18}F$</TEX> leads to changes in a classical neurotransmitter, tryptophan, in both the brains of control goldfish and goldfish contaminated by irradiation.
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