Abstract
While the mechanisms regulating hematopoietic stem cells (HSCs) are increasingly understood, less is known about the impact of environmental factors on this process. Estrogenic compounds were isolated as modifiers of runx1 expression in a zebrafish chemical screen. Exposure to 17beta-estradiol (E2) significantly decreased runx1+ HSCs in the AGM compared to controls by in situ hybridization and qPCR (p<0.01) which was confirmed by CD41 FACS (p<0.0001). E2 treatment from 12-24 hours post-fertilization disrupted vessel formation and angiogenic sprouting, as indicated by flk1 (kdrl) and altered the assignment of ephrinb2+ arterial identity.
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