178-POS

Abstract

Objectives A hypoxic placenta may release factors that result in maternal endothelial dysfunction, among which, leptin seems to participate. The aim of this study was evaluate the association of leptin levels in placenta, maternal and umbilical cord plasma between normotensive controls and pre-eclamptic women. Methods Placental biopsies, maternal and umbilical cord plasma were taken from 67 normotensive and 50 pre-eclamptic women. Leptin levels were quantified using MagPlexTH-C – microspheres system. The leptin concentration was analyzed by ANCOVA adjusted by BMI, gestational age and maternal age. To estimate the difference between groups, mean ratio (MR) and confidence interval (CI) of 95% was calculated. Analysis between leptin levels and maternal/fetal variables were made by Pearson correlation. The null hypothesis was rejected when p Results Higher levels of leptin were found in maternal plasma (MR = 1.40; 95%CI: 1.00–1.97, p = 0.049) and placenta (MR = 1.82; 95%CI: 1.11–2.98, p = 0.019) in patients with pre-eclampsia. A positive correlation between gestational age, birth weight vs. fetal leptin levels in pre-eclamptic group was found ( r = 0.416, r = 0.618; p r = 0.36, p r = 0.344, p = 0.021). No correlation was found between placenta vs. fetal plasma or fetal plasma vs. maternal plasma. Conclusions Leptin values in patients with pre-eclampsia were significantly increased in maternal plasma and placental tissue. Besides that, the positive correlation not observed between placentae and maternal leptin levels in pre-eclamptic group may be due to a loose of this regulation in pre-eclampsia, and should be considered for future work. Finally, a strong positive correlation in relation to clinical data and fetal leptin concentration, in pre-eclamptic group, intensify the possibility of leptin being also involved in preterm birth and birth weight, when pre-eclampsia is presented. Disclosures M.R. Hentschke: None. A.B. Comparsi: None. L.S. Lucas: None. F. Sontag: None. G. Gadonski: None. L.G. Paula: None. C.E. Poli-de-Figueiredo: None. B.E. Pinheiro da Costa: None.