Abstract
Open column chromatography is an effective and common technique for the separation and purification of chemical constituents. Limonoids are found in significant quantities in citrus fruits. Citrus limonoids have documented anti-cancer activity in several types of cancer, such as breast, colon, skin, and neuroblastoma in animal models and in vitro cell culture studies. Furthermore, limonoids have shown anti-inflammatory properties and inhibitory effects on bone resorption. In addition to many potential health benefits, limonoids have also shown antifungal and insect anti-feedant properties. To meet the large demand of limonoids for bioactivity studies, defatted grapefruit seeds were extracted using acetone and concentrated under vacuum. The dried extract was loaded onto a silica gel column and eluted with mixtures of dichloromethane and ethyl acetate with increasing polarity to obtain three compounds. The purity of the compounds (1–3) have been analyzed by HPLC and the structures have been identified by using NMR spectra and mass spectra as nomilin, limonin, and deacetylnomilin, in respective order of elution. The results will be presented in greater detail on the poster. This project is based upon work supported by the USDA-CSREES under Agreement USDA IFAFS #2001 52102 02294 and USDA #2005-34402-14401 “Designing Foods for Health” through the Vegetable & Fruit Improvement Center.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
