174 BONE MARROW MESENCHYMAL STROMAL CELL THERAPY FOR EXTERNAL URETHRAL SPHINCTER RESTORATION IN A RAT MODEL OF STRESS URINARY INCONTINENCE

Abstract

You have accessJournal of UrologyStem Cell Research1 Apr 2011174 BONE MARROW MESENCHYMAL STROMAL CELL THERAPY FOR EXTERNAL URETHRAL SPHINCTER RESTORATION IN A RAT MODEL OF STRESS URINARY INCONTINENCE Oleg Loutochin, Lysanne Campeau, Nicoletta Eliopoulos, Manaf Bouchentouf, Bertil Blok, Jacques Galipeau, and Jacques Corcos Oleg LoutochinOleg Loutochin Montreal, Canada More articles by this author , Lysanne CampeauLysanne Campeau Montreal, Canada More articles by this author , Nicoletta EliopoulosNicoletta Eliopoulos Montreal, Canada More articles by this author , Manaf BouchentoufManaf Bouchentouf Montreal, Canada More articles by this author , Bertil BlokBertil Blok Montreal, Canada More articles by this author , Jacques GalipeauJacques Galipeau Montreal, Canada More articles by this author , and Jacques CorcosJacques Corcos Montreal, Canada More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.243AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES To assess the effect of intra-sphincteric injections of bone marrow mesenchymal stromal cells (MSCs) on Valsalva leak point pressure (VLPP) changes in an animal model of stress urinary incontinence (SUI). METHODS 24 female Sprague-Dawley rats underwent bilateral pudendal nerve section for SUI induction. Six rats were SUI controls, 6 received periurethral injection of Plasma-Lyte (SUI placebo control) and 12 were given periurethral injection of PKH26-labelled MSCs. Four weeks after injection, conscious cystometry was undertaken in animals and VLPP recorded. All groups were sacrificed, and frozen urethra sections were submitted to pathology assessment. RESULTS Rat MSCs were positive for the cell surface antigens CD44, CD73, CD90, and RT1A, and negative for CD31, CD45, and RT1B, confirming their stem cell phenotype. In vitro, differentiated MSCs expressed α-smooth muscle actin (SMA) and desmin, markers of smooth and striated muscles. Immunohistochemistry of rat urethras revealed PKH26-labelled MSCs in situ and at the injection site. LPP was significantly improved in animals infused with MSCs. Mean LPP was 24.28 ± 1.47 cm H20 in rats transplanted with MSCs and 16.21 ± 1.26 cm H20 in SUI controls injected with Plasma Lyte (p<0.001). CONCLUSIONS Bone marrow rat MSCs have the ability to differentiate and skew their phenotype towards smooth and striated muscles, as demonstrated by SMA up-regulation and desmin expression. Periurethral injection of MSCs in an animal model of SUI restored the damaged external urethral sphincter and significantly improved VLPP. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e72-e73 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Oleg Loutochin Montreal, Canada More articles by this author Lysanne Campeau Montreal, Canada More articles by this author Nicoletta Eliopoulos Montreal, Canada More articles by this author Manaf Bouchentouf Montreal, Canada More articles by this author Bertil Blok Montreal, Canada More articles by this author Jacques Galipeau Montreal, Canada More articles by this author Jacques Corcos Montreal, Canada More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...