17α,20β,21-trihydroxy-4-pregnen-3-one and 17α,20β-dihydroxy-4-pregnen-3-one stimulated spermiation in protandrous black porgy, Acanthopagrus schlegeli

Abstract

The objective of this study was to investigate the effects of sex steroids on spermiation in protandrous male black porgy, Acanthopagrus schlegeli. Experiments on common carp (Cyprinus carpio) were also conducted for comparison. Fifty male black porgy were divided into 5 groups and injected with a superactive analogue of mammalian luteinizing hormone releasing hormone (LHRH-A), 17α,20β,21-trihydroxy-4-pregnen-3-one (20β-S), 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DHP), 11-ketotestosterone (11-KT) or saline. The dosage of the sex steroids given on days 0, 2, 4 and 6 was 330, 330, 990 and 1980 µg kg-1 body weight, respectively. Milt volume and sperm concentrations were measured on days 0, 2, 4, 6, 8 and 10. Similar treatments were also conducted in 45 male common carp. Milt volume was significantly increased in black porgy after treatment with 20β-S and 17α,20β-DHP; 17α,20β-DHP had stimulatory effects on spermiation at a lower dose (900 µg kg-1 body weight, p < 0.05) as compared to 20β-S (1980 µg kg-1 body weight, p < 0.01). In the common carp, milt volume was also increased after treatment with LHRH-A and 17α,20β-DHP but not with 20β-S. 17α,20β-DHP stimulated spermiation at a lower dose in common carp (330 µg kg-1 body weight) than in black porgy (990 µg kg-1 body weight). However, 11-KT did not stimulate spermiation in black porgy or common carp. The concentrations of plasma 11-KT could immediately reflect to the administration of exogenous 11-KT in black porgy.